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KMID : 0379520110270020071
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한국독성학회지
2011 Volume.27 No. 2 p.71 ~ p.76
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Inhibition of p65 Nuclear Translocation by Baicalein
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Seo Min-Bum
Lee Seog-Ki
Jeon Young-Jin
Im Jin-Su
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Abstract
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We demonstrate that baicalein, a bioactive flavonoid originally isolated from Scutellaria baicalensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of peritoneal macrophages and RAW 264.7 cells with baicalein inhibited LPS-stimulated nitric oxide production in a dose-related manner. Immunohistochemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression in RAW 264.7 cells. Immunostaining of p65, EMSA, and reporter gene assay showed that baicalein inhibited NF-κB nuclear translocation, DNA binding, and transcriptional activation, respectively. Collectively, these series of experiments indicate that baicalein inhibits iNOS gene expression by blocking NF-κB nuclear translocation. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of baicalein on iNOS suggest that baicalein may represent a useful anti-inflammatory agent.
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KEYWORD
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Baicalein, Macrophages, iNOS, NF-κB
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